The isolation of rhodanese from Pseudomonas aeruginosa by affinity chromatography.
نویسندگان
چکیده
Rhodanese (thiosulphate : cyanide sulphur-transferase; EC 2 . 8 . I . I) was originally ,described by Lang (1933). The enzyme catalyses the formation of thiocyanate from cyanide and thiosulphate according to the reaction : S20!+ CN-+ SCN+ SO:-. Rhodanese activity has been demonstrated in most mammalian tissues, with the greatest activity present in liver and kidney. The enzyme has been purified from liver (Horowitz & DeToma, 1970) and kidney (Westley & Green, 1959) as well as from several micro-organisms (Bowen, Butler & Happold, 1965; McChesney, 1958; Smith & Lascelles, 1966; Tabita, Silver & Lundgren,
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ورودعنوان ژورنال:
- Journal of general microbiology
دوره 103 1 شماره
صفحات -
تاریخ انتشار 1977